Restrictions in EthoVision XT

In the following table you find a summary of the main restrictions in EthoVision XT.

General

Feature

Maximum number

Notes

Subjects per arena

1 (Base module), up to 16 (Social Interaction module)

The limit of 16 subjects is set by the software. It may be difficult to discriminate between 16 different color marks depending on the light conditions. See Color markers

We recommend to only use Tracking from video files when you track multiple subjects and multiple body points.

The technical limit of 16 subjects per arena can be overridden in the software; contact Noldus for the possible solutions. Contact the Noldus Support

Arenas

100

With the Multiple Arena Module; One arena with the Base Module.

Arenas must not overlap. The number of arenas does not depend on the number of cameras. You can have for example 96 wells in one camera image, or 16 arenas with 16 camera views (one arena per camera).

When you combine tracking of multiple subjects (with colors) and multiple arenas, performance may limit the total number of subjects followed simultaneously.

Trials

10000

 

Behavior types scored manually

256

 

Hardware commands and signals

1024

With the Trial and Hardware Control Module

Arena Settings cache

50 MB

 

Heatmap dimensions (pixels)

65536

 

Rows or columns in analysis results table

One million

 

Free intervals

2000

See Free interval

Time bins

No restriction

If you create more than 1000 time bins, the group charts are not displayed.

 

Computer

Feature

Limitations

Notes

Computer type

Workstation

We recommend the use of Dell workstations especially when using multiple add-on modules (e.g. tracking nose-, center- and tail-base points in multiple subjects simultaneously)

When tracking for more than 24 hours, restart the PC before a new trial.

See Hardware

Video graphics card

NVIDIA, 4 GB or larger

If you opt to buy your own PC, it must have a stand-alone graphics card that supports Direct3D acceleration, We recommend NVIDIA video cards with a memory of 4 GB or larger.

For tracking subjects with Deep learning, there are further restrictions. See Deep learning: Requirements

Processor and RAM memory

Intel

We recommend an Intel processor; for desktop PCs, a 32 GB RAM, and for laptops a 16 GB RAM.

See also

System requirements: Hardware

Ordering a PC from another supplier

More information on graphics cards (GPUs)

 

Video resolution, sample rate and trial duration

Feature

Maximum

Notes

Video resolution (camera image)

Depends on camera type

See Cameras supported by EthoVision XT

Video resolution (video file)

1900 x 1200

Higher resolutions, for example 4K video may work. Test a few sample videos and check for missing samples. Keep DDS selected in the Acquisition screen.

See DDS (Detection determines speed)

Sample rate (live tracking)

60 frames per second; it depends on the camera

See Cameras supported by EthoVision XT

Sample rate (tracking from video file)

Not specified

EthoVision XT can handle short video files with a frame rate of 1000, but that also depends on the video format. Test a few sample videos and check for missing samples before running the experiment.

Trial duration

72 hours, but this depends on the tested setup

See Resolution, frame rate, and maximum trial duration

important  When tracking from video files at high resolution and/or frame rate, always select DDS in the Playback Control window. This way EthoVision XT adjust the analysis speed to the time needed to analyze each frame without skipping frames. See DDS (Detection determines speed)

important  When tracking for more than 24 hours, always reboot the PC after every trial to refresh the PC resources, the RAM specifically. Failure to do so could result in crashing, which can corrupt the experiment, or the recorded video.

 

Applications and add-ons

Feature

Maximum number

Notes

Multiple Body Point module (live tracking)

Four arenas per computer

In principle it is possible to track in more than 4 arenas, but it depends on the apparent size of the subjects in the video image (in pixels).

Sample rate must be 25 samples/s or higher.

Multiple Arena module

100 arenas

The number of arenas may be limited by other factors, for example the number of subjects in each arena and the tracking of multiple body points.

Contact Noldus if you wish to work with more than 100 arenas.

Social Interaction module

16 subjects per arena

If subject are unmarked, there may be subject identity swaps.

CognitionWall (Sylics)

Four arenas, only center-point tracking; up to 72 h recording

In case of recordings > 24 h, we advise to restart the PC after each trial. A workstation is required.

Behavior Recognition

Subjects: one per arena

Arenas: one (live tracking), up to four (offline tracking)

Requires the Multiple Body Point module. Camera must be overhead. Camera frame rate must be at least 25 fps with no missing frames. Cannot work together with Deep learning-based tracking. The PC must be a workstation.

See Behavior Recognition: Requirements

Deep learning for nose-tail base tracking

One or two subjects per arena (up to 4 arenas)

See Deep learning: Requirements

 

See also

Choose your camera

System requirements

The test environment